Akademik Veri Yönetim Sistemi
Turkish Journal of Agriculture and Forestry, cilt.24, sa.4, ss.491-498, 2000 (SCI-Expanded)
Desiccation of somatic embryos under different air humidities conditioned by saturated salt solutions for different durations; relationships between desiccation and germination or root formation in the dark; and effects of desiccation and gibberellic acid on germinating of 'Su-2' walnut somatic embryonic embryo line were investigated. Survival of embryos was low (about 10%) or zero when loss of fresh weight was about 90% and dry matter was about 70%. The highest germinating percentages (13.3-23.3%) were obtained with about 40.4-77.3% of loss of fresh weight and 9.1-34.6% of dry matter of desiccated embryos in covered plates over saturated MgCl2.6H2O for 4 days, Mg(NO3)2.6H2O for 5 days or NaCl for 5-6 days, and with uncovered plates over ZnSO4.7H2O for 6-7 days or NH3SO4 for 6-8 days. The highest rooting percentage (80.9%) in the dark were obtained with 56.2% of loss of fresh weight and 16.8% of dry matter of desiccated embryos in covered plates over saturated MgCl2.6H2O for 4 days. To improve germination, both desiccation and GA3 treatments were more effective than only desiccation or GA3 treatments on germination. Germinating was best (46.0%) with desiccation followed by incubation in the dark for 15 days prior to being transferred to a medium containing 9 mg/l GA3 in the light.