Detection and identification of Toxocara canis in infected dogs using PCR

Oge H., Öge S., Ozbakis-Beceriklisoy G.

HELMINTHOLOGIA, cilt.56, sa.2, ss.118-123, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 56 Sayı: 2
  • Basım Tarihi: 2019
  • Doi Numarası: 10.2478/helm-2019-0008
  • Dergi Adı: HELMINTHOLOGIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.118-123
  • Anahtar Kelimeler: Toxocara canis, dog, faecal examination, ATPase subunit-6, STRAY DOGS
  • Ankara Üniversitesi Adresli: Evet

Özet

Faecal samples were collected from 224 dogs (47 villages) in Ankara. Toxocara spp. eggs were diagnosed in faeces using centrifugal flotation and sedimentation methods. A total of 21 dogs (9.38 %) were positive for Toxocara spp. eggs. In this study, we used the PCR technique that, in combination with DNA sequencing, allows the detection and identification of T. canis eggs in faeces of infected dogs. For this purpose, the ATPase subunit-6 gene (mtDNA) was selected as a target for the amplification T. canis. The primers were used to amplify 217 bp region. Amongst 21 coproscopically detected Toxocara isolates from dogs, 5 (23.8 %) samples were PCR-positive for T. canis, and the remaining 16 samples were PCR-negative. Results indicate that PCR can detect Toxocara canis DNA in faeces of infected dogs, but efficacy was low when compare to sedimentation/flotation. PCR is additional test for diagnosing of this infection. But, the difficulties of identifi cation based on PCR in faecal examinations need to be investigated further.