Utilization of olive mill wastewater for selective production of lipids and carotenoids by Rhodotorula glutinis

Keskin A., Ünlü A. E., Takaç Z. S.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, cilt.107, sa.15, ss.4973-4985, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 107 Sayı: 15
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1007/s00253-023-12625-x
  • Dergi Adı: APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ABI/INFORM, Applied Science & Technology Source, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Compendex, Computer & Applied Sciences, EMBASE, Environment Index, Food Science & Technology Abstracts, Geobase, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.4973-4985
  • Anahtar Kelimeler: Carotenoid, microbial lipid, olive mill wastewater, Rhodotorula glutinis, torularhodin, OLEAGINOUS YEAST, GROWTH, TORULARHODIN, GLYCEROL, STRAINS, ACID, ACCUMULATION, EXTRACTION, SUBSTRATE, TORULENE
  • Ankara Üniversitesi Adresli: Evet

Özet

Olive mill wastewater (OMW) is a zero-cost substrate for numerous value-added compounds. Although several studies on the production of lipids and carotenoids by Rhodotorula glutinis in OMW exist, none of them has specifically focused on the conditions for a target lipid or carotenoid. This study presents cultivation conditions that selectively stimulate the cell biomass, individual carotenoids and lipids. It was found that supplemental carbon and nitrogen sources as well as illumination affected cell biomass the most. High temperature, low initial pH, illumination, lack of urea and presence of glycerol stimulated the lipid synthesis. The highest total lipid content obtained in undiluted OMW supplemented with urea was 11.08 +/- 0.17% (w/w) whilst it was 41.40 +/- 0.21% (w/w) when supplemented with glycerol. Moreover, the main fatty acid produced by R. glutinis in all media was oleic acid, whose fraction reached 63.94 +/- 0.58%. Total carotenoid yield was significantly increased with low initial pH, high temperature, illumination, certain amounts of urea, glycerol and cultivation time. Up to 192.09 +/- 0.16 mu g/g cell carotenoid yield was achieved. Torularhodin could be selectively produced at high pH, low temperature and with urea and glycerol supplementation. To selectively induce torulene synthesis, cultivation conditions should have low pH, high temperature and illumination. In addition, low pH, high temperature and urea supplementation served high production of beta-carotene. Up to 85.40 +/- 0.76, 80.67 +/- 1.40 and 39.45 +/- 0.69% of torulene, torularhodin and beta-carotene, respectively, were obtained under selected conditions.