Visible light–crosslinked histamine-functionalized hyaluronic acid and gelatin hydrogels for wound healing applications
Polymer Bulletin, cilt.83, sa.9, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 83 Sayı: 9
- Basım Tarihi: 2026
- Doi Numarası: 10.1007/s00289-026-06521-3
- Dergi Adı: Polymer Bulletin
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core, Chimica, Compendex, INSPEC, Academic Search Ultimate (EBSCO), Engineering Source (EBSCO), Materials Science & Engineering Collection (ProQuest), Technology Collection (ProQuest)
- Anahtar Kelimeler: Gelatin, Histamine, Histamine-conjugated, Hyaluronic acid, Photo-crosslink, Visible light
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Ankara Üniversitesi Adresli: Evet
Özet
Histamine-functionalized hyaluronic acid (HA) and gelatin (Gel) hydrogels were developed via covalent conjugation and visible light–mediated Ru/SPS photopolymerization for potential wound healing applications. Histamine was successfully grafted onto HA and Gel backbones via EDC/NHS coupling, and hydrogel networks were formed under 460 nm visible light irradiation. Structural confirmation was achieved through FTIR and ¹H-NMR analyses, while the degree of substitution (DS) was determined to be approximately 11% for HA–His conjugates. The prepared hydrogels exhibited rapid swelling behavior, reaching equilibrium within 120 min and attaining approximately 1.5-fold weight increase. Biodegradation studies revealed an initial mass loss of ~20–25% within 5 days in PBS, while enzymatic degradation with lysozyme resulted in accelerated degradation beginning at day 3. Rheological analysis demonstrated shear-thinning behavior with a dominant elastic response (G′ > G″), indicating structural stability suitable for injectable and 3D bioprinting applications. In vitro cytocompatibility assays (MTT and Alamar Blue) showed no significant cytotoxicity (p > 0.05), while scratch assay results indicated enhanced wound closure rates of 98.30% (HA–His) and 98.44% (Gel–His) after 24 h compared to control groups. SEM analysis revealed interconnected porous architectures (~4–50 μm for HA–His and ~15–45 μm for Gel–His), favorable for cell infiltration and nutrient diffusion. Overall, this study presents a covalently stabilized, visible light–crosslinked histamine-functional hydrogel platform with tunable physicochemical properties and promising in vitro wound healing performance. However, further in vivo and inflammation-specific studies are required to fully validate its clinical translational potential.