IN VITRO SELECTION OF DROUGHT TOLERANT REGENERANTS IN DURUM WHEAT (TRITICUM DURUM DESF.)

BENLİOĞLU B., Ozgen M.

APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, cilt.19, sa.3, ss.1813-1825, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19 Sayı: 3
  • Basım Tarihi: 2021
  • Doi Numarası: 10.15666/aeer/1903_18131825
  • Dergi Adı: APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.1813-1825
  • Anahtar Kelimeler: drought stress, polyethylene glycol, endosperm-supported embryo culture, Triticum durum, Triticum dicoccum, PLANT-REGENERATION, WATER-STRESS, CALLUS INDUCTION, CULTIVARS, EXPOSURE, GROWTH, YIELD
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, a total of 12 genotypes were used, including 1 hulled emmer and 11 registered ones which are important as genetic resources and for durum wheat cultivation. The responses of these genotypes to different drought stress levels were determined in vitro. In this research, to create drought stress, high molecular weight polyethylene glycol (PEG 6000) was used. In the trials, 5 different PEG 6000 doses were administered to induce drought stress with rates of 0 (control),-0.50 bar,-1.48 bar,-2.95 bar and-4.91 bar. During the experiments, after the callus was formed with the endosperm supported mature embryo culture, regeneration capacity and plantlets they formed were evaluated by applying different levels of drought stress to the callus. Using these parameters, stress tolerance index, stress sensitivity index and tolerance index values of durum wheat genotypes were calculated. According to the results, there was a significant decrease in all parameters examined with the increase of drought stress. While Artuklu and Saricanak-98 cultivars had the highest drought tolerance, cakmak-79, cesit-1252, Eminbey and Kunduru-1149 cultivars were sensitive to drought and Triticum dicoccum, which is an important gene source, was also sensitive.