Research Information System
21st International Congress of Chemical and Process Engineering, CHISA 2014 and 17th Conference on Process Integration, Modelling and Optimisation for Energy Saving and Pollution Reduction, PRES 2014, Prague, Czech Republic, 23 - 27 August 2014, vol.4, pp.2519-2521
In this study, the alcohol dehydrogenase (ADH) activities of several vegetables and asymmetric reduction of acetophenone using vegeables as biocatalysts were investigated. Four species of fresh vegetables such as carrot (Daucus carota), radish (Raphanus sativus), potato (Ipomoea batatas) and grape (Vitis) were applied to find an ideal biocatalyst for the asymmetric reduction reaction. Primarily, the specific activity of the ADH present in the homogenate was determined UV spectrophotometrically. Specific activities of (ADH) in the homogenates of D. carota, R. sativus, I. batatas and vitis were measured respectively as 16 (U/mL), 6 (U/mL), 2 (U/mL), 10.04 (U/mL). Then, carrot r oot was utilized as biocatalyst for asymmetric reduction. Freshly cut vegetables pieces (10 g) and acetophenone (2mM) were added into 250 mL conical flask including 50 mL of 0.1 mM sodium phosphate buffer pH=7. The reaction was incubated in an orbital shaker at 150 rpm and 33 °C temperature. The progress of the reaction was monitored by HPLC with chiral column. The best results, in term of the conversion (c) and enantimeric excess (e.e), were obtained with fresh carrot (D. carota) as 100% e.e. and 92% conversion.