Adventitious Shoot Regeneration in Grasspea (Lathyrus sativus L.)

ŞAHİN DEMİRBAĞ N., KENDİR H., Assim M.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, cilt.14, sa.3, ss.297-302, 2008 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 3
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1501/tarimbil_0000001044
  • Dergi Adı: JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.297-302
  • Anahtar Kelimeler: Grass pea, plant regeneration, adventive shoot regeneration, rooting, thidiazuron (TDZ), CHICKPEA CICER-ARIETINUM, PLANT-REGENERATION, HIGH-FREQUENCY, THIDIAZURON, INDUCTION, EXPLANTS, CYTOKININ
  • Ankara Üniversitesi Adresli: Evet

Özet

Grasspea is an important and high yielding forage species in providing quality roughage necessary for livestock. The study aimed to develop a rapid and reliable regeneration protocol for grass pea. The cotyledon node explants were excised from the in vitro grown seedlings fo grasspea and cultuired on MS medium containing different concentrations of TDZ. TDZ has structure that does not contain purine and is therefore more effective compared to number of other cytokinines. The study reports maximum shoot regeneration frequency of 100% on MS medium containing 0.2 mg/l TDZ. Maximum number of 11.83 and 10.56 shoots per explant were recorded on MS medium containing ile 0,2 mg/L TDZ ve 300 mg/L Casein hdyrolsate and 0.2 mg/l TDZ respectively. No statisitical difference was recorded among all regeneration medium for shoot length. Sixty percent rooting was achieved by pulse treating regenerated shoots with 100mg/l IBA for 5 and 7.5 minutes. In conclusion, a successful regeneration protocol was developed in grass pea using cotyledon node explant in a very short time period.