Akademik Veri Yönetim Sistemi
Fabad Journal of Pharmaceutical Sciences, cilt.43, sa.3, ss.143-150, 2018 (Scopus)
© 2018 Society of Pharmaceutical Sciences of Ankara (FABAD). All rights reserved.Obesity is the most common nutritional disorder and associated with an increased risk of developing insulin resistance and type 2 diabetes. There have been several reports related with inflammation in diabetes and obesity, but the relationship between inflammatory status, lipid profile and aldose reductase (AR) enzyme activity in diabetic and high-fat diet (HFD) is not evaluated before. The objective of the present study is to determine the AR activity, cytokine (tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6)), cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), triglyceride and atherogenic index levels in control, diabetes and HFD groups and evaluate the correlation between them. Blood glucose levels and body weights of the Wistar albino rats were monitored at the 1st and at 6th weeks of the diabetes and HFD groups. AR is isolated from rat livers and AR activities of these groups were determined by a spectrophotometric method. Cytokine levels were measured by ELISA. Blood total cholesterol, HDL, LDL and triglyceride levels were measured by colorimetric diagnostic kits. The AR enzyme activity is 0.249 U/L in control group, 0.389 U/L in diabetes and 0.356 U/L in HFD group (p≤0.05). Increased TNF-α and IL-6 levels were detected in both diabetes and HFD groups. Serum cholesterol, LDL, triglyceride and atherogenic index levels were also found higher in diabetes and HFD groups. Enhancement of AR enzyme activities correlated with the increased cytokine levels and also with corrupted lipid profiles in both diabetes and HFD groups. HFD might have similar pathophysiological mechanisms that result in several similar metabolic complications like diabetes.