Akademik Veri Yönetim Sistemi
European Wound Mangagement Association 2023, Milan, İtalya, 3 - 05 Mayıs 2023, ss.1
Aim: The aim of this
study is to produce hybrid dermal grafts containing human skin extracellular
matrix (HS-ECM) using 3-dimensional (3D) bio-printing technology.
Method: The dermis
layer of the human skin tissue obtained from aesthetic surgery operations was
separated from epidermis by enzymatic reactions and removed from its cells
using four different decellularization protocols. The most effective protocol
was selected according to DNA content, histological analysis and FTIR spectrums
of ECM. HS-ECM based hydrogel (1%) with alginate (2%) and gelatin (7%) was used
as an ink for 3D bio-printing. The characterization of 3D hybrid graft was
evaluated in terms of mechanical strength, swelling and biodegradability. For
biocompatibility analysis, human keratinocyte cells seeded on 3D hybrid graft
were analyzed under laser scanning confocal microscope using Live-Dead and
F-actin-DAPI staining.
Results / Discussion: The protocol number IV which
reduced the amount of DNA, exhibited normal tissue integrity, and similar collagen
and glycosaminoglycan contents compared to natural tissue was chosen. FTIR
spectrums of natural tissue and HS-ECM were displayed similar patterns. Following
the 3D bio-printing, 0.480 mm and 5-layer grid prints were obtained. They exhibited
a maximum tensile strength of 4 kPa, and an elongation of 10% with a strain
value of 0.1. 3D hybrid grafts preserved
their structural integrity in 7 days, and the keratinocyte cells were able to
survive for 14 days and shown their normal morphology.
Conclusion: The present
study suggests that the fabricated 3D hybrid grafts have potential to be used
for regenerative and reconstructive medicine applications.