Genotypic correlation between Salmonella Enteritidis isolates from broiler breeders and hatchery flocks

Müştak İ. B., Müştak H. K., Sarıçam İnce S.

ACTA VETERINARIA-BEOGRAD, vol.71, no.4, pp.392-402, 2021 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 71 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.2478/acve-2021-0033
  • Journal Name: ACTA VETERINARIA-BEOGRAD
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Central & Eastern European Academic Source (CEEAS), EMBASE, Food Science & Technology Abstracts, Veterinary Science Database, Directory of Open Access Journals
  • Page Numbers: pp.392-402
  • Keywords: multilocus sequence typing, phylogeny, pulsed field gel electrophoresis, Salmonella, FIELD GEL-ELECTROPHORESIS, ENTERICA SEROVAR TYPHIMURIUM, TANDEM REPEAT ANALYSIS, CONTAMINATION, NUMBER, SURVEILLANCE, COMBINATION, RESISTANCE, POULTRY, STRAINS
  • Ankara University Affiliated: Yes

Abstract

In this study, Salmonella Enteritidis strains isolated from dust and environmental materials from different flocks located in Turkey's Western Black Sea region were examined by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). A total of 59 S. Enteritidis strains isolated from broiler breeder and hatchery flocks, and one S. Enteritidis strain isolated from a stool sample of a farm worker were examined. PFGE analysis revealed two major PFGE groups and nine different macro restriction profiles. It was determined that 85% (51/60) of the strains were close to each other and comprised Group I. All S. Enteritidis strains had the same sequence type (ST): ST11. Isolation of strains with a single genotype suggests that there may be a cross transmission between the flocks.