Chemical profiles and anti-inflammatory activity of the essential oils from seseli gummiferum and seseli corymbosum subsp. corymbosum

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Tosun A., Chun J., Jerkovic I., Marijanovic Z., Fenu M. A., Aslan S. S., ...Daha Fazla

Natural Product Communications, cilt.11, sa.10, ss.1523-1526, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 10
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1177/1934578x1601101025
  • Dergi Adı: Natural Product Communications
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1523-1526
  • Anahtar Kelimeler: Apiaceae, Seseli, Essential oil, COX-2, iNOS, NF-kappa B, NITRIC-OXIDE SYNTHASE, NF-KAPPA-B, RAW-264.7 MACROPHAGES, COX-2 EXPRESSION, INHIBITION, COUMARINS, TURKEY, RESINOSUM, APIACEAE, INOS
  • Ankara Üniversitesi Adresli: Evet

Özet

The anti-inflammatory activity of the essential oils from Seseli corymbosum subsp. corymbosum Pall. ex Sm. (SC) and Seseli gummiferum Boiss. and Heldr. subsp. corymbosum (SG) was investigated for the first time on lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. The main constituents (determined by GC-FID and GC-MS analyses) were germacrene D (54.1%) and sabinene (22.4%) in SG oil and β-phellandrene (29.2%), α-phellandrene (8.2%) and germacrene D (2.5%) in SC oil. SC and SG oils inhibited nitric oxide (NO) production with IC50 values of 56.1 and 108.2 μg/mL, respectively. The oils also inhibited prostaglandin E2 (PGE2) with IC50 values of 49.4 μg/mL (SC oil) and 95.5 μg/mL (SG oil). The inhibitory effect of SC and SG oils was accompanied by dose-dependent decreases of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expressions in LPS-induced RAW 264.7 cells. The research of the reporter gene assay on nuclear factor κB (NF-κB) showed that SC and SG oils inhibited NF-κB transcriptional activity. The obtained results suggest that SC and SG oils exert the anti-inflammatory effects in LPS-stimulated RAW 264.7 cells by suppressing NF-κB activation.