Evaluation of Antropogenic Aquatic Toxicity by and Alternative and New Approach; using PLHC-1 and RTG-2 cell lines and CYP1A1 Biomarker

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Dikmeni B. Y., AYDIN F. G., TUTUN H., SEVİN S.

KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE, cilt.23, sa.1, ss.247-258, 2020 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.18016/ksutarimdoga.vi.556762
  • Dergi Adı: KSU TARIM VE DOGA DERGISI-KSU JOURNAL OF AGRICULTURE AND NATURE
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.247-258
  • Anahtar Kelimeler: RTG2, PLHC, CYP1A1, Cytotoxicity, Ankara Stream, POLYCYCLIC AROMATIC-HYDROCARBONS, RAINBOW-TROUT, BIOCHEMICAL MARKERS, PRIMARY CULTURES, P450 INDUCTION, NEUTRAL RED, FISH, ASSAY, CYTOCHROME-P4501A, CYTOTOXICITY
  • Ankara Üniversitesi Adresli: Evet

Özet

Permanent organic pollutants (POPs) are chemicals that can remain intact in nature for a long time and affect the health of humans, animals and the environment. In this study, cytotoxicity and 7-Ethoxyresorufin O-deethylase (EROD) activity of water (6 stations) and sediment (12 stations) samples collected from Ankara Stream for 12 months were evaluated on fish gonadal (RTG-2) and fish hepatocellular carcinoma (PLHC-1) cell lines. Cytotoxic activity were found to vary according to the sampling region and period. This study, provide important information for the methodology used in the detection of environmental contaminants and evaluation of pollution from environmental samples using sensitive, cheap, fast and trustable method by cytotoxicity and EROD activity in fish cells. It is recommended that these results could be optimized and used by legal authorities for risk evaluation