Akademik Veri Yönetim Sistemi
7th Trends in Medical Mycology, Lisbon, Portekiz, 9 - 12 Ekim 2015, cilt.58, sa.427, ss.51-226, (Özet Bildiri)
Objective: Denture
stomatitis is a widespread infection of completedenture users. The condition is
related to multiple factors such asusing old dentures, denture trauma and
insufficient oral hygiene. Among all of these factors, the candidal biofilm
formed on tissue surfaces of dentures are believed to be the primary
etiological factorof denture stomatitis. Various types of candida species are
contributed in this process, while Candida albicans is believed to be the most
important one. Because of this, to prevent and to treat denture stomatitis, it
is believed that elimination of C. albicans biofilm with various types of
disinfection procedures is of paramount importance. Recently, in addition to
conventional microwave andsodium hypochlroide disinfection regimes, strong and
weak acidic electrolyzed waters are introduced. Regarding to this information, this
study evaluated the effect of different disinfection regimes on C.albicans
biofilm which is formed on acrylic denture base resin, in vitro.
Methods: The samples were
subjected to the previously mentioneddisinfection process. At this stage,
samples were transferred into 200 ml of sterile distilled water for the
microwave disinfection at 650 W for 3 min. The chemical disinfection method was
carried outtransferring each sample into 1 ml disinfection solution. The
disinfection process was carried out with weak and strong electrolyzed
acidwater and sodium hypochlorite for 1, 1 and 10 min, respectively. The
disinfection was not adjusted on the control group. The C. albicans ATCC (90128)
suspensions were subcultured intofresh medium and incubated at 37 °C for 24 h.
After 24 h of incubation, liquid cultures were washed three times. The optical
density ofthe yeast stock solution was adjusted to a 0.5 MacFarland solution. 1
ml yeast suspension was incubated with the sample for 48 h at 37 °C in a
horizontal shaker. To remove non-adhering fungi, all samples were carefully
washed three times in PBS. Samples taken from the experimental group and
control group were transferred into1 ml of PBS and vortexed for 60 sec. to
resuspend any microorganisms present. 0.1 ml of this resuspensions was plated
on Sabourauddextrose agar and incubated aerobically for 48 h at 37 °C. After incubation,
the microbial colony count of each plated denture wasquantified by the eye
counting method and the logarithm of colonyforming units (CFU) per ml was then
calculated. Results All four types of disinfection methods are completely eliminated
C. albicans biofilm on acrylic resin specimens.
Conclusion: This results
revealed that electrolyzed acid waters areeffective against C. albicans biofilm
and they can be used to disinfectdentures for the patients with denture
stomatitis.