Comparison of seeds of Colchicum speciosum and Gloriosa superba in respect to colchicine and colchicoside contents by RP-LC

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Gümüştaş M., Polat D., Kılıç C. S., Akalin K., Özkan S. A., Coskun M.

Natural Product Communications, cilt.11, sa.3, ss.397-400, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 11 Sayı: 3
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1177/1934578x1601100319
  • Dergi Adı: Natural Product Communications
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.397-400
  • Anahtar Kelimeler: HPLC, Colchicum speciosum, Gloriosa superba, Colchicine, Colchicoside
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, colchicine (CLN) and colchicoside (CLS) contents of the methanolic extracts of the seeds of Colchicum speciosum Steven that were collected from Uzungõl, Trabzon and also the seeds belonging to two different samples of Gloriosa superba Linn. imported from India were compared by using RP-LC (Reversed Phase High Pressure Liquid Chromatography). This proposed method is advantageous in terms of sample preparation and selective separation of the compounds. Also the method was successfully validated in accordance with the International Conference on Harmonization (ICH) guideline acceptance criteria for system suitability, linearity and range, precision, specificity and accuracy. As a conclusion of this analysis, the colchicoside and colchicine contents of G. Superba (GSI), G. Superba (GSII) and C. Speciosum (CS) were found to be 312.9 mg/100 g and 333.1 mg/100 g; 434.0 mg/100 g and 471.1 mg/100 g, and 51.9 mg/100 g and 75.9 mg/100 g, respectively.