Transcriptome analysis and potential mechanisms of bovine oocytes under seasonal heat stress

ÖZMEN Ö., Karaman K.

Animal Biotechnology, vol.34, no.4, pp.1179-1195, 2023 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 4
  • Publication Date: 2023
  • Doi Number: 10.1080/10495398.2021.2016429
  • Journal Name: Animal Biotechnology
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Compendex, EMBASE, Environment Index, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.1179-1195
  • Keywords: Heat stress, cows, oocyte, fertility, bioinformatics analysis, DEVELOPMENTAL COMPETENCE, REPRODUCTION, DISRUPTION, EXPRESSION, PATHWAYS, HOLSTEIN, GENES, SHOCK
  • Ankara University Affiliated: Yes

Abstract

© 2021 Taylor & Francis Group, LLC.Heat stress is the major factor affecting cattle fertility but molecular mechanisms of deleterious impacts of elevated temperature on oocyte are still not well known. Therefore, the aim of this study is to gain a better understanding of the underlying molecular mechanism of how heat stress affects GV-stage and MII-stage oocytes and discover hub genes to heat resistance for cow oocytes. In this study, we used the bioinformatics approach to discover the differentially expressed genes between GV-stage and MII-stage oocytes, which were collected during spring and summer. When GV-stage oocytes were compared to MII-stage oocytes collected in July (Jul DEGs group) a total of 1068 genes were found as differentially expressed as a result of heat stress. Also, HSPA8, COPS5, POLR2L, PSMC6, and TPI1 were identified as the common top ranked genes for the Jul DEGs group. The highest connected hub gene for the Jul DEGs group was determined as HSPA8. Our results showed that different heat response mechanisms might be activated to protect oocytes from elevated temperatures in cattle. The identified genes and their associated pathways might play an important role in the response to heat stress that affects the oocytes in cattle.