Use of a multiplex-polymerase chain reaction for detection of Salmonella and Chlamydophila psittaci from caged birds

SAREYYÜPOĞLU B., Cantekin Z.

ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.56, sa.4, ss.269-273, 2009 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 56 Sayı: 4
  • Basım Tarihi: 2009
  • Dergi Adı: ANKARA UNIVERSITESI VETERINER FAKULTESI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.269-273
  • Anahtar Kelimeler: Caged birds, Chlamydophila psittaci, identification, multiplex-PCR, Salmonella spp., CHLAMYDIA-PSITTACI, RAPID DETECTION, FECAL SAMPLES, PCR
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, development of a multiplex-PCR (m-PCR) assay for identification of Salmonellosis and Psittacosis in caged birds was aimed. DNAs extracted from Salmonella spp. strains from the collection of our department and Salmonella spp. isolates from caged birds in addition to the extracted DNA samples belong to two different Chlamydophila psittaci clinical strains (strain 6BC and a strain isolated from a parakeet), constituted the material of the study. A m-PCR assay was developed which use iroBF and iroBR primers to specifically amplify 606 bp sequence of iroB gene of Salmonella spp. DNA in the same reaction mix and with the same conditions, CpsiA and CpsiB primers were used to amplify 300 bp sequence of pmp gene of C. psittaci. The specificity and the sensitivity of the assay were determined by using positive controls (Salmonella and Chlamydia DNA samples). We believe that the newly developed m-PCR assay is a fast, reliable and an economic assay, which has the potential tor direct identification of Salmonella spp. and C. psittaci from the clinical samples.