The effects of cisplatin and jaceosidin on SH-SY5Y neuroblastoma cells: an electron microscopic, molecular and biochemical study

BAYRAM P., AKSAK KARAMEŞE S., oezdemir B., DURAK A., BİLLUR D.

ULTRASTRUCTURAL PATHOLOGY, 2023 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/01913123.2023.2218911
  • Dergi Adı: ULTRASTRUCTURAL PATHOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, EMBASE, MEDLINE
  • Anahtar Kelimeler: Cisplatin, Electron microscopy, Jaceosidin, Oxidative stress, SH-SY5Y, Western blotting, BREAST-CANCER CELLS, UP-REGULATION, PLATINUM COMPOUNDS, OXIDATIVE STRESS, RAT MODEL, APOPTOSIS, AUTOPHAGY, EXPRESSION, ANTIOXIDANT, INHIBITION
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, our aim was to show both the single and combined effects of cisplatin and jaceosidin in SHSY-5Y neuroblastoma cells. For this purpose, we used MTT cellular viability assay, Enzyme-Linked Immunosorbent Assay (ELISA), Transmission Electron Microscopy (TEM), Immunofluorescence Staining Assay (IFA) and Western blotting (WB) assay. According to MTT findings, IC50 dose was detected as 50 mu M cisplatin and 160 mu M jaceosidin co-application. Therefore, experimental groups were finally selected as control, cisplatin, 160 mu M jaceosidin and Cisplatin +160 mu M jaceosidin. Cell viability was decreased in all groups, and the IFA findings confirmed the viability analysis. WB data indicated that matrix metalloproteinase 2 and 9 levels, as indicators of metastasis, decreased. While LPO and CAT levels increased in all treatment groups, it was observed that the activity of SOD decreased. When TEM micrographs were investigated, cellular damages were determined. In the light of these results, it can be said that cisplatin and jaceosidin have a potential to increase the effects of each other synergistically.