Akademik Veri Yönetim Sistemi
CHROMATOGRAPHIA, cilt.77, sa.1-2, ss.99-107, 2014 (SCI-Expanded)
To support real biological sample application, a simple, selective and rapid LC-MS method has been developed and validated for the sensitive determination of metoclopramide in rabbit blood, ex vivo permeation studies and pharmaceutical dosage form. LC-MS analysis was performed isocratically on a Zorbax SB-C-18 column with a mobile phase consisting of methanol:ammonium acetate buffer (pH 3.0) 75:25 (v/v) at a flow rate of 0.70 mL min(-1). The outlet of the column was connected to a single quadrupole mass spectrometer with positive mass spectrometric detection. Ions were detected in the positive multiple reaction monitoring mode. The assay was linear over the concentration range of 1.25-200 pg mu L-1 with a limit of detection of 0.077 pg mu L-1 for standard solutions and 2.5-200 pg mu L-1 with a limit of detection of 0.42 pg mu L-1 for serum samples. The method is applicable, covering a variety of pharmaceutical and biological studies. Metoclopramide was extracted from rabbit blood by liquid-liquid extraction using ether as the extraction solvent. The reproducibility of the method was found to be between 0.96 and 1.98 % (RSD) values. The proposed method has been extensively validated for the determination of metoclopramide in all working media. The sample preparations, flow rate and run time of the analytical systems are not time consuming. Moreover, for the stability of metoclopramide, the effect of temperature, UV light, H2O2, HCl and NaOH were also investigated.