Application of ELISA and HAI test for the detection of mumps virus antibodies

ALTANLAR N., Akin A.

Acta Pharmaceutica Turcica, cilt.40, sa.3, ss.141-144, 1998 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 40 Sayı: 3
  • Basım Tarihi: 1998
  • Dergi Adı: Acta Pharmaceutica Turcica
  • Derginin Tarandığı İndeksler: Scopus, International Pharmaceutical Abstracts
  • Sayfa Sayıları: ss.141-144
  • Anahtar Kelimeler: ELISA, Hemagglutination Inhibition Test, Mumps Virus
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, Mumps specific antibodies were investigated with ELISA and HAI test in 405 sera obtained from different age groups. Specific viral antigen was prepared with Mumps virus which was produced in vero cell. The control antigen was prepared in the same way. In ELISA, indirect technique was applied by using polystyrene microtitre plates. Antigen, control antigen and serum dilutions were determined by using block titrations. 405 sera samples belonging to different age groups, specific antibodies first were tested by HAI test. In this test 347 out of 405 sera (85.7%) were proven seropositive, 58 (14.3%) sera were not found positive. In total, using ELISA, out of 405 sera samples, 349 (86.2%) IgG were found positive and 16 (4%) of those were found to be IgM seropositive, 56 (13.8%) sera were found seronegative.