Akademik Veri Yönetim Sistemi
ACTA CHIMICA SLOVENICA, sa.4, ss.558-565, 2024 (SCI-Expanded)
Leuprolide acetate is a synthetic gonadotropin-releasing hormone agonist used in the treatment of central precocious puberty, uterine fibroids, endometriosis, and prostate cancer. In this study, the rapid, simple, environmentally friendly, and sensitive voltammetric method was developed for the quantification of leuprolide acetate in the presence of an anionic surfactant on a glassy carbon electrode. The effect of surfactant types, pH, and scan rate on the voltammetric response of leuprolide acetate were evaluated. Using square wave voltammetry, two anodic peaks were observed at 0.81V and 1.26 V for the glassy carbon electrodes at pH 3.70 (acetate buffer solution). The oxidation peak currents of leuprolide acetate were enhanced in the presence of 8.0 x 10-2 mM sodium dodecyl sulfate. The sodium dodecyl sulfate monomer can be drawn to amino moieties in the drug structure through electrostatic interaction. The voltammetric behavior of leuprolide acetate exhibited irreversible and diffusion-adsorption mix-controlled processes by cyclic voltammetry. All stripping conditions and square wave voltammetric parameters were optimized at pH 3.21 (phosphate buffer). Under optimized conditions, the proposed method exhibits linearity in the concentration range of 3.64 x 10-6-2.00 x 10-4 mM with a nano-level detection limit of 4.70 x 10-7 mM by square wave adsorptive stripping voltammetry. The developed method was applied for the determination of leuprolide acetate in a pharmaceutical dosage form with a satisfactory recovery of 97.72%.