Incidence of spiromesifen resistance and resistance mechanisms in Tetranychus urticae populations collected from strawberry production areas in Turkey

İNAK E., Alpkent Y. N., Cobanoglu S., TOPRAK U., Van Leeuwen T.

CROP PROTECTION, cilt.160, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 160
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1016/j.cropro.2022.106049
  • Dergi Adı: CROP PROTECTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Environment Index, Veterinary Science Database
  • Anahtar Kelimeler: Spiromesifen, Resistance mechanisms, Copy number, P450s, 2-SPOTTED SPIDER-MITE, SPIROTETRAMAT RESISTANCE, INSECTICIDE RESISTANCE, ACARI TETRANYCHIDAE, BIOLOGICAL-CONTROL, CROSS-RESISTANCE, SPIRODICLOFEN, EXPRESSION, CARBOXYLASE, STRAIN
  • Ankara Üniversitesi Adresli: Evet

Özet

Tetranychus urticae Koch is a serious pest of strawberries and its remarkable ability to develop resistance has often caused failure in chemical control. In the present study, resistance to spiromesifen was detected in two out of nine field-populations of T. urticae collected from important strawberry production areas in Turkey, with resistance ratio's mounting to 60-and 140-fold. Synergism assays showed the involvement of detoxification enzymes, especially cytochrome P450 monooxygenases, in resistance against spiromesifen in field-collected T. urticae populations. We further selected the most resistant field-collected population (POP14) with spi-romesifen and obtained an extremely resistant population (LC50 > 5000 mg a.i./L). Gene expression analysis with qPCR supported the involvement of cytochrome P450s in spiromesifen resistance in this selected popula-tion. In addition, we also detected a higher expression of acetyl-CoA carboxylase (ACCase), the target-site of spiromesifen, in the spiromesifen-selected population. Furthermore, a F1640L mutation in the conserved region of CT-domain of ACCase was uncovered in the most resistant field populations, but the role of this mutation in spiromesifen resistance needs further validation. Overall, spiromesifen still seems to be an effective tool in the control of T. urticae in strawberry production areas of Turkey, but resistance is on the rise and should be considered a serious threat.