Integrative Analysis of Serial CosMx Spatial Transcriptomic Sections Reveals Tissue Architecture and Immune Cell Types

Uyar R., Yılmazer Aktuna A., Zoccheddu M.

4th Atlanta Workshop for Single-cell Omics, Georgia, Amerika Birleşik Devletleri, 16 - 17 Nisan 2025, ss.1-2, (Özet Bildiri)

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Georgia
  • Basıldığı Ülke: Amerika Birleşik Devletleri
  • Sayfa Sayıları: ss.1-2
  • Ankara Üniversitesi Adresli: Evet

Özet

We integrated spatial transcriptomics data from two tonsil slides (1AU and 2AU) using Field

of View (FOV) alignment and deep learning–based spatial registration. A subset of 25,000

cells was annotated with SingleR using the HCATonsilData reference, enabling high-

resolution mapping of cell types across sections. Comparing single-slide and integrated data

revealed improved resolution and more robust cell-type identification within the tonsil

microenvironment.