Comparative Study of the Quantitative Resolution of Paracetamol and Methocarbamol Mixture by Spectrophotometry with Wavelet Transform and UPLC Techniques

GÜZEL R., ERTEKİN ÖZKAN Z. C., ZİYADANOĞULLARI B., DİNÇ E., ZİYADANOĞULLARI R.

PHARMACEUTICAL CHEMISTRY JOURNAL, cilt.55, sa.10, ss.1126-1132, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 55 Sayı: 10
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s11094-021-02547-1
  • Dergi Adı: PHARMACEUTICAL CHEMISTRY JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, EMBASE
  • Sayfa Sayıları: ss.1126-1132
  • Anahtar Kelimeler: spectrophotometry, wavelet transform, ultra-performance liquid chromatography, paracetamol, methocarbamol, CHROMATOGRAPHIC METHOD, HUMAN PLASMA, VALIDATION
  • Ankara Üniversitesi Adresli: Evet

Özet

The aim of this study was to develop a new simple and cost-effective spectrophotometric method to be used as an alternative to chromatography for the analysis of paracetamol and methocarbamol (PAR-MET) combination. In this regard, a comparative study on the quantitative resolution of PAR and MET mixture using continuous wavelet transform (CWT) and ultra-performance liquid chromatography (UPLC) techniques was performed. In interpretation of the spectrophotometric data, three different wavelet families Biorthogonal3.5 (Bior3.5), Mexican Hat Function (Mexh), and Symlets5 (Sym5) were found suitable to get better transform of zero-order spectra of the samples containing PAR and MET. The concentrations of PAR and MET in the working range of 4.0 - 20.0 mu g/mL and 5.0 - 45.0 mu g/mL, respectively, were proportional to the wavelet amplitudes. This mathematical relationship was used to construct calibration curves for each compound. After validation of the CWT method, analyses of commercial tablets were successfully performed. A new UPLC method was developed and validated in order to compare the results with those obtained by the CWT method and the data were found comparable. The analysis of results obtained from experimental trials showed that the proposed CWTmethod is suitable for the quality control and quantitative assessment of both active substances in tablet formulations.