Akademik Veri Yönetim Sistemi
CHROMATOGRAPHIA, cilt.59, sa.9-10, ss.555-560, 2004 (SCI-Expanded)
A simple, rapid, specific, sensitive HPLC method has been developed for the determination of piroxicam in the tablet dosage form and in human plasma. The method totally eliminates solvent extraction and time-consuming separation procedures. Plasma proteins were precipitated by addition of 3:1 (v/v) acetonitrile-methanol, ZnSO4, and MgSO4 and the supernatant was injected directly on to a 250 mm x 4.6 mm, 5 mum particle Spherisorb analytical column. Acetonitrile-methanol-0.04 mol L-1 KH2PO4, 40:10:50 (v/v); PH 3.8, was used as mobile phase. The drug was detected by UV detection at 330 nm. The response was linear over the range of 0.01-10 mug mL(-1) and 0.025-5 mug mL(-1) in mobile phase and human plasma samples, respectively. The proposed method was used without interference from the endogenous substances, for determination of piroxicam in plasma samples obtained from healthy volunteers. The results revealed that the method would be useful in monitoring plasma levels of the drug during pharmacokinetic studies. Assay of piroxicam in its dosage forms for quality-control purposes could also be performed successfully by use of this method.