Improvement of superoxide dismutase activity using experimental design and radical promoters

Unlue A. E., Takac S.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, vol.31, no.5, pp.1046-1054, 2017 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 5
  • Publication Date: 2017
  • Doi Number: 10.1080/13102818.2017.1353923
  • Journal Name: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1046-1054
  • Keywords: Glycerol, Rhodotorula glutinis, superoxide dismutase, response surface methodology, radical promoters, OXIDATIVE STRESS-RESPONSE, RECOMBINANT ASPERGILLUS-NIGER, RHODOTORULA-GLUTINIS, BETA-CAROTENE, DEBARYOMYCES-HANSENII, HYDROGEN-PEROXIDE, YEAST, CATALASE, CULTURE, CELLS
  • Ankara University Affiliated: Yes

Abstract

The culture conditions of Rhodotorula glutinis were optimised to obtain high activity of superoxide dismutase (SOD) enzyme, which is an important member of the antioxidant defence system. By the use of glycerol as a carbon source, which is a by-product of biodiesel production, 2.40U of SOD activity was achieved under the optimised conditions found by Central Composite Design (pH = 5.47, T = 33.9 degrees C and 16.50 g L-1 glycerol). In order to provide a further increase in SOD activity, different types of radical promoters were tested and the highest enzyme activity was achieved with methyl viologen, 21.57 U mg(-1) protein with a 1.6-fold increase. The effect of dissolved oxygen (DO) on SOD activity was also investigated employing different operational strategies in bioreactors. SOD activity was found to be induced only when high DO concentration was supplied starting from the beginning of the cultivation where the activity of 75 U mg(-1) protein was obtained.