Akademik Veri Yönetim Sistemi
European Wound Mangagement Association 2024, London, İngiltere, 1 - 03 Mayıs 2024, ss.1
Aim: This study aims to isolate
bacteria originating from human skin microbiota and determine their potential antibacterial
effects against clinically important skin pathogens.
Method: Swab samples
from healthy human skin (n=22) were plated on MRS agar and incubated until
colonies were observed. From each plate, phenotypically distinct colonies (n=102)
were picked onto fresh media. Their antibacterial activity was determined by
agar well-diffusion assay against clinically important skin pathogens. The most
effective isolate which exhibited a remarkable inhibitory effect against all
tested pathogens was selected and identified by molecular methods. Postbiotics of
the isolate were obtained following the fermentation process. The minimum inhibitory concentration (MIC) against all
tested pathogens was determined by microtiter plate assay. Anti-biofilm
activity was evaluated by crystal violet staining. Quorum sensing-related gene
expressions were analyzed using RT-PCR.
Results /
Discussion: The selected isolate was identified Staphylococcus
hominis according to its 16S rRNA gene region (1.492 bp) sequencing. Postbiotics displayed an antibacterial effect
against all tested pathogens and their sub-MIC value eradicated their biofilm
production. It was also proven that the gene expression levels related to the quorum
signal transduction system were significantly down-regulated in a
dose-dependent manner.
Conclusion: This
study highlights the potential effects of postbiotics against important skin pathogens
and demonstrates their multifaceted roles in wound healing.