Polymorphisms of cytochrome P450 1A1, glutathione S-transferases M1 and T1 in a Turkish population

Ada A., SÜZEN H. S., Iscan M.

TOXICOLOGY LETTERS, cilt.151, sa.1, ss.311-315, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 151 Sayı: 1
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1016/j.toxlet.2003.12.075
  • Dergi Adı: TOXICOLOGY LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.311-315
  • Anahtar Kelimeler: cytochrome P450 1A1, genetic polymorphism, glutathione S-transferase M1, glutathione S-transferase T1, Turkish population, S-TRANSFERASE M1, BLADDER-CANCER, GENE POLYMORPHISM, CYP1A1 MUTATIONS, NULL GENOTYPE, COLON-CANCER, GSTM1, SUSCEPTIBILITY, GSTT1, RISK
  • Ankara Üniversitesi Adresli: Evet

Özet

Intra-ethnic as well as inter-ethnic differences are known to exist in the frequencies of cytochromeP450(CYP)1A1, glutathione S-transferase (GST)M1, and GSTT1 gene polymorphisms with which associations have been shown for several cancers. In this study, CYP1A1 m2, GSTM1, and GSTT1 gene polymorphisms were determined among 133 healthy individuals of a Turkish population. On the basis of polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) methodology, the frequency of CYP1A1 m2 mutation was determined. The multiplex PCR protocol was used to determine the frequency of the deleted genotypes of both GSTM1 and GSTT1 genes. The frequencies of Ile/Ile (wild type), Ile/Val (heterozygous variant), and Val/Val (homozygous variant) CYP1A1 m2 genotypes were 90.2%, 9.8%, and 0%, respectively. The frequencies of the deleted GSTM1 (null) and GSTT1 (null) genotypes were 51.9% and 17.3%, respectively. These results show that the frequencies of the CYP1A1 m2, GSTM1, and GSTT1 gene polymorphisms in a Turkish population are similar to Caucasian populations. (C) 2004 Elsevier Ireland Ltd. All rights reserved.