Assay of ephedrine hydrochloride and theophylline in pharmaceutical formulations by differential-derivative spectroscopy

Erk N.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, cilt.23, sa.2-3, ss.255-261, 2000 (SCI-Expanded, Scopus) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 23 Sayı: 2-3
  • Basım Tarihi: 2000
  • Doi Numarası: 10.1016/s0731-7085(00)00288-0
  • Dergi Adı: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.255-261
  • Anahtar Kelimeler: theophylline, ephedrine hydrochloride, differential-derivative spectrophotometry, pharmaceutical formulations, SPECTROPHOTOMETRIC DETERMINATION, LIQUID-CHROMATOGRAPHY, SODIUM
  • Ankara Üniversitesi Adresli: Evet

Özet

Rapid and accurate binary mixture resolution of ephedrine hydrochloride and theophylline was performed. Differential-derivative spectrophotometry with a zero-crossing measurement technique was used for the quantitative determination of ephedrine hydrochloride and theophylline in pharmaceuticals. Neither sample pretreatment nor separation were required. Linear calibration graphs of differential first derivative values (at 262.3 and 256.3 nm for theophylline and ephedrine hydrochloride, respectively) versus concentration (in the ranges 6.0-40.0 and 100.0-1000.0 mu g ml(-1) for theophylline and ephedrine hydrochloride, respectively) were obtained with negligible intercepts. Vierordt's method was also developed for a comparison method. Commercial tablet and laboratory-prepared mixtures containing both drugs were assayed using the developed methods. Both methods showed good linearity, precision and reproducibility. (C) 2000 Elsevier Science B.V. All rights reserved.