Akademik Veri Yönetim Sistemi
Hacettepe University Journal of the Faculty of Pharmacy, cilt.29, sa.2, ss.95-104, 2009 (Scopus)
Quantitative determination and stability test of sulfamethoxazole and trimethoprim in oral suspension were carried out by spectrophometric classical least square calibration method. Stability test of the related veterinary drugs was performed by using the following conditions: Room temperature, refrigerator and etuve (37 0C) for each 3 month during 12 months. For the spectrophotometric analysis, the standard series of sulfamethoxazole and trimethoprim was prepared in the concentration range of 2.0-11.0 μg/mL and 3.0-18.0 μg/mL respectively. The absorbances of standard series were measured at three-wavelength set (237, 257 and 288 nm). A classical least square calibration was obtained by using the absorptivities at three-wavelength set. The validity of the spectral method was done by analyzing the synthetic binary mixtures. Afterwards the method was successfully applied to simultaneous analysis and stability test of the commercial veterinary formulation containing sulfamethoxazole and trimethoprim.