The effect of injectable platelet-rich fibrin in combination with autogenous bone grafts on bone healing in an ovariectomized osteoporotic rat model with critical-sized defects

Durmuşlar M. C., Gülşen E. A., Bayburt K. A., Ballı U., OCAK M., FIRAT A., ...Daha Fazla

Saudi medical journal, cilt.45, sa.8, ss.791-798, 2024 (Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 45 Sayı: 8
  • Basım Tarihi: 2024
  • Doi Numarası: 10.15537/smj.2024.45.8.20240224
  • Dergi Adı: Saudi medical journal
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.791-798
  • Anahtar Kelimeler: animal experiment, bone regeneration, osteoporosis, platelet rich fibrin
  • Ankara Üniversitesi Adresli: Evet

Özet

OBJECTIVES: To assess the impact of autogenous graft materials, including autogenous bone graft (ABG) and injectable platelet-rich fibrin (I-PRF) clots, on bone defect regeneration in ovariectomized osteoporotic rats. METHODS: Wistar rats (6-8 weeks old) were ovariectomized, and surgery began after 8 weeks. A 5-mm defect was created bilaterally in the parietal bones of 16 rats, which were divided into 4 groups. Group A (blank) had untreated defects; group B had defects filled with 0.1 ml of I-PRF, group C had defects filled with 0.1 ml of ABG, and group D had defects filled with a combination of 0.1 ml each of I-PRF and ABG. Four weeks post-surgery, the rats were euthanized. Bone regeneration was evaluated through histopathologic analysis and microcomputed tomography (micro-CT). RESULTS: In ovariectomized rats treated with ABG or I-PRF, bone regeneration was enhanced, with increased periosteal activity, osteoblast count, and new bone volume, as determined histologically. The ABG+I-PRF group had the highest periosteal vascularity, but the difference compared to the ABG group was not statistically significant (p>0.05). Osteoblast numbers were significantly higher in the ABG+I-PRF group than in the blank group (p<0.05). Micro-CT showed the highest mean new bone volume ratio in the ABG+I-PRF group, followed by the ABG group. CONCLUSION: The combined use of ABG and I-PRF enhances bone formation in osteoporotic rats following ovariectomy.