Akademik Veri Yönetim Sistemi
Fabad Journal of Pharmaceutical Sciences, cilt.50, sa.2, ss.371-384, 2025 (Scopus, TRDizin)
Dexketoprofen (DEX) and paracetamol (PAR), a common drug pair in multimodal analgesia, exhibit overlapping absorbance spectra, making conventional UV-Vis spectroscopy unsuitable for their simultaneous quantification. The objective of this work was to develop and validate chemometrics-assisted spectrophotometric methods for the simultaneous quantification of these drugs in effervescent tablet formulations, despite their overlapping spectra. UV-Vis spectrophotometry was combined with Principal Component Regression (PCR) and Partial Least Squares (PLS) regression to develop predictive models. A calibration set of 25 binary mixtures, with concentration ranges of 3–18 μg/mL for DEX and 5–25 μg/mL for PAR, was used to develop the chemometric models. The models were built using the concentration data set and the spectral data between 220 and 320 nm (Δλ=0.1 nm). The accuracy and precision of the proposed chemometric methods were assessed by analyzing a set of independent test samples as well as intra-day and inter-day samples. The PCR and PLS models provided accurate and precise quantification, with mean recovery values and relative standard deviations within acceptable limits. Commercial effervescent tablet samples were analyzed to evaluate the applicability, and assay results showed good agreement with label claims. The proposed PCR and PLS methods offer reliable and cost-effective alternatives to HPLC for the simultaneous analysis of DEX and PAR in pharmaceutical formulations. These methods are suitable for routine quality control, reducing analysis time and solvent consumption.