Development of a Citrus-Based Perfume Formulation and Evaluation of Its Anti-Breast Cancer Activity
56th INTERNATIONAL SYMPOSIUM ON ESSENTIAL OILS (ISEO 2026)), Alpes-Maritimes, Fransa, 6 - 09 Eylül 2026, ss.93, (Özet Bildiri)
- Yayın Türü: Bildiri / Özet Bildiri
- Basıldığı Şehir: Alpes-Maritimes
- Basıldığı Ülke: Fransa
- Sayfa Sayıları: ss.93
- Ankara Üniversitesi Adresli: Evet
Özet
Conventional synthetic perfumes may contain chemical ingredients associated with adverse health effects, including potential carcinogenicity and endocrine disruption (Alblooshi, 2025). In contrast, natural perfume formulations prepared from essential oils are considered safer alternatives due to their plant-derived bioactive constituents and lower synthetic chemical content. The present study aimed to characterize the chemical profile of a citrus-based perfume formulation using Gas Chromatography–Mass Spectrometry (GC–MS) analysis and to investigate its potential anticancer activity against MDA-MB-231 breast cancer cells. The perfume formulation was prepared using commercially available essential oils with dominant citrus aromatic characteristics. Chemical characterization of the formulation was performed by GC–MS analysis to identify major volatile secondary metabolites. The cytotoxic activity of the perfume formulation against MDA-MB-231 breast cancer cells was evaluated using the MTT cell viability assay. Cells were treated with different concentrations of the perfume formulation (100, 10, 1, and 0.1 µg/mL) for 24 h, and cell viability was determined spectrophotometrically according to mitochondrial metabolic activity. GC–MS analysis identified 31 different constituents in the perfume formulation. The major compounds were 1,2-propanediol (18.9%), linalool (14.4%), and linalyl acetate (12.0%). The perfume formulation exhibited concentration-dependent cytotoxic activity, as cell viability decreased from 96.8% ± 1.89 at 0.1 µg/mL to 19.5% ± 2.23 at 100 µg/mL. Cell viability values at 1 and 10 µg/mL were determined as 40.6% ± 6.43 and 28.0% ± 2.39, respectively. The observed cytotoxic activity against MDAMB-231 breast cancer cells suggests that citrus-based perfume formulations enriched with bioactive essential oil constituents may possess additional functional value beyond their aromatic properties. This activity may be associated with the presence of major compounds such as linalool and linalyl acetate, which have previously been reported to exhibit antioxidant and anti-inflammatory effects in various cancer models (Wu et al., 2025). Therefore, the demonstrated anticancer potential may increase both the pharmaceutical and commercial significance of such natural perfume formulations.