Akademik Veri Yönetim Sistemi
Ankara Universitesi Veteriner Fakultesi Dergisi, cilt.56, sa.1, ss.25-30, 2009 (SCI-Expanded)
The present study was undertaken to determine the morphology, histochemical properties, localization and quantitative distribution of Paneth cells in the small intestine of the Angora rabbit. Tissue samples taken from the duodenum, jejunum and ileum of 10 healthy adult Angora rabbits, obtained from private breeders, constituted the material of the study. The Paneth cells, which were determined to be located within the crypts of Lieberkuhn, were identified on their basally located nucleus and apically located acidophilic granules. These granules gave positive reactions with Mallory's triple staining technique as well as with the application of Phloxine-tartrazine, Alcian blue-performic acid and Mallory's phosphotungstic acid-hematoxylin method. Staining with periodic acid-Schiff (PAS), Alcian blue (pH 2.5), and PAS-Alcian blue pH 2.5 gave negative reactions. Paneth cells were determined not to display a uniform distribution throughout the small intestine and cell numbers were ascertained to show a gradua l increase from the duodenum towards the ileum. The difference between the three regions of the small intestine was determined to be statistically significant (p<0.01). Electron microscopic examination revealed the presence of electron-dense and homogenous granules in the apical cytoplasm of some Paneth cells, whereas homogenous granules of different electron density existed in some other cells.