Influence of emulsifiers on the formation and in vitro anticancer activity of epirubicin loaded PLGA nanoparticles

Esim O., Bakirhan N. K., Sarper M., Savaser A., Ozkan S. A., Ozkan Y.

Journal of Drug Delivery Science and Technology, cilt.60, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 60
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.jddst.2020.102027
  • Dergi Adı: Journal of Drug Delivery Science and Technology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Biotechnology Research Abstracts, EMBASE
  • Anahtar Kelimeler: Nanoparticle, Surfactant, Epirubicin, Sodium cholate, CONTROLLED-RELEASE, PACLITAXEL, DELIVERY, NANOSPHERES, FORMULATION, FABRICATION, SURFACTANT, TPGS, PVA
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2020 Elsevier B.V.Surfactants play an important role in preparation of nanoparticles. In this study, it was aimed to investigate the effect of surfactant type and concentration on development of epirubicin loaded Poly (D,L-lactide-coglycolide acid) (PLGA) nanoparticles. In this regard PVA, TPGS, sodium cholate and Poloxamer 188 were used as surfactants to prepare epirubicin loaded PLGA nanoparticles in three concentrations. It was found that the particle size of nanoparticles was varied in the range 109.6 ± 5.19 nm and 511.5 ± 23.99 nm. The size of nanoparticles was smaller when sodium cholate was used (p < 0.05). It was observed that encapsulation efficiency of nanoparticles was higher for those emulsified by sodium cholate at low concentrations (0.3%) (p < 0.05). Lower IC50 doses were found lower at 24 h when nanoparticles fabricated by sodium cholate (4.71 ± 0.67 μg/mL, 17.96 ± 1.25 μg/mL and 27.78 ± 1.44 μg/mL for 0.3%, 1% and 2%) compared with drug solution (218.00 ± 20.33 μg/mL) in human lung squamous cell carcinoma (SK-MES-1) cells (p < 0.05). Both qualitative fluorescent images and quantitative results showed that nanoparticles prepared with sodium cholate demonstrated greater cellular uptake of nanoparticles. Thus, sodium cholate could be useful in developing epirubicin loaded PLGA nanoparticles for non-small cell lung cancer treatment.