Akademik Veri Yönetim Sistemi
TROPICAL PLANT PATHOLOGY, cilt.47, sa.3, ss.421-429, 2022 (SCI-Expanded)
Tomato is among the most produced and exported products in greenhouse cultivation in Turkey. Tomato leaf mold is one of the most important fungal diseases that can cause significant yield losses in greenhouse and hydroponic tomato cultivation. In this study, the mating types of 97 Fulvia fulva isolates obtained from different provinces in Turkey were determined. We found that 43 isolates were MAT1-1 type and 54 isolates were MAT1-2 type. Both mating types of the pathogen were determined in the same greenhouse. The distribution of mating types conformed to the ratio of 1:1. This showed the high probability of sexual reproduction of Turkish F. fulva populations. In addition, a multiplex PCR method was developed for the rapid detection of pathogen mating types. In multiplex PCR results, except for the main amplified fragment, 806 and 613 bps were obtained in MAT1-1 type, while fragment with a length of 613 bp was obtained in MAT1-2 type. It is thought that the mating types of the pathogen can be determined by using these DNA regions showing homology in multiplex PCR since the fragment sizes obtained with mating type primers are very close to each other. The reliability of the method was supported by the band profiles obtained by cutting PCR products with the PstI enzyme. This will be helpful in mating-type studies of F. fulva.