Dose-, treatment- and time-dependent toxicity of superparamagnetic iron oxide nanoparticles on primary rat hepatocytes

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Gokduman K., Bestepe F., Li L., Yarmush M. L., Usta O. B.

NANOMEDICINE, vol.13, no.11, pp.1267-1284, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 11
  • Publication Date: 2018
  • Doi Number: 10.2217/nnm-2017-0387
  • Journal Name: NANOMEDICINE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1267-1284
  • Keywords: superparamagnetic iron oxide nanoparticles (SPION), primary rat hepatocytes, albumin, urea, reactive oxygen species (ROS), IN-VITRO, COLLAGEN SANDWICH, GENE TRANSFECTION, OXIDATIVE STRESS, VIVO TOXICITY, DELIVERY, DRUG, HEPATOTOXICITY, MECHANISMS, CELLS
  • Ankara University Affiliated: No

Abstract

Aim: As a first study in literature, to investigate concentration-dependent (0-400 mu g/ml) and exposure-dependent (single dosing vs cumulative dosing) effects of superparamagnetic iron oxide nanoparticles (d = 10 nm) on primary rat hepatocytes in a time-dependent manner. Materials & methods: Sandwich-cultured hepatocyte model was used to evaluate viability, hepatocyte specific functions and reactive oxygen species level. Results: In terms of all parameters, generally statistically more significant effects were observed in a concentration- and time-dependent manner. In terms of hepatocyte-specific functions, cumulative dosing caused significantly (p < 0.05) more deleterious effects at 48th hour. Conclusion: A combination of various biomarkers should be employed for the evaluation of the effect of superparamagnetic iron oxide nanoparticles on liver, and each biomarker should be analyzed in a time- and exposure-dependent manner.