Molecular characterization of picobirnaviruses in small ruminants with diarrhea in Turkey

KARAYEL HACIOĞLU İ., Gul B., ALKAN F.

VIRUS GENES, cilt.58, sa.3, ss.238-243, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 58 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s11262-022-01894-9
  • Dergi Adı: VIRUS GENES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, CAB Abstracts, Chemical Abstracts Core, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.238-243
  • Anahtar Kelimeler: Picobirnavirus, Genogroup I, Molecular characterization, RdRp, Sheep, GENOGROUP-I, VIRUSES, GASTROENTERITIS, IDENTIFICATION, ROTAVIRUS, ALIGNMENT, SEGMENTS, SAMPLES
  • Ankara Üniversitesi Adresli: Evet

Özet

Picobirnaviruses (PBVs), detected in a wide range of host species, are viruses of which limited information is available about their pathogenic potential, ecology, or evolutionary characteristics. In this study, a molecular analysis of segment 2 encoding the PBV RNA-dependent RNA-polymerase (RdRp) in small ruminants with diarrhea in Turkey was undertaken. A total of 66 fecal samples or gut contents from diarrheic small ruminants including 55 sheep and 11 goats were screened. Four samples (6.06%), obtained from sheep in different farms, yielded the expected amplicon size for the genogroup I RdRp gene fragment, whereas no positivity was detected for genogroup II PBVs. Phylogenetic analysis revealed high levels of genetic diversity among the genogroup I PBVs. Additionally, all PBV infected sheep were also positive for rotavirus A. This study, reporting the presence of the PBVs in sheep Turkey for the first time, contributes to the molecular characterization and epidemiology of PBVs.