Carboxymethylcellulose-gelatin-superoxidase dismutase electrode for amperometric superoxide radical sensing

Kocabay O., EMREGÜL E., Aras S., EMREGÜL K. C.

BIOPROCESS AND BIOSYSTEMS ENGINEERING, cilt.35, sa.6, ss.923-930, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 35 Sayı: 6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s00449-011-0677-x
  • Dergi Adı: BIOPROCESS AND BIOSYSTEMS ENGINEERING
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.923-930
  • Anahtar Kelimeler: Carboxymethylcellulose-gelatin, Superoxide dismutase, Biosensor, Superoxide anions, Scavengers, IMMOBILIZED CYTOCHROME-C, OXYGEN FREE-RADICALS, PULSE RADIOLYSIS, PROTEIN OXIDATION, IN-VIVO, BIOSENSOR, ANION, ANTIOXIDANTS, DISEASE, SYSTEM
  • Ankara Üniversitesi Adresli: Evet

Özet

A novel, highly sensitive superoxide dismutase biosensor for the direct and simultaneous determination of superoxide radicals was developed by immobilization of superoxide dismutase within carboxymethylcellulose-gelatin on a Pt electrode surface. The parameters affecting the performance of the biosensor were investigated. The response of the CMC-G-SOD biosensor was proportional to O (2) (center dot-) concentration and the detection limit was 1.25 x 10(-3) mM with a correlation coefficient of 0.9994. The developed biosensor exhibited high analytical performance with wider linear range, high sensitivity and low response time. The biosensor retained 89.8% of its sensitivity after use for 80 days. The support system enhanced the immobilization of superoxide dismutase and promoted the electron transfer of superoxide dismutase minimizing its fouling effect. The biosensor was quite effective not only in detecting O (2) (center dot-) , but also in determining the antioxidant properties of acetylsalicylic acid-based drugs and the anti-radical activity of healthy and cancerous human brain tissues.