High sensitivity detection of salivary 8-hydroxy deoxyguanosine levels in patients with chronic periodontitis

Kurgan Ş., OEnder C., Altingoz S. M., BAĞIŞ N., Uyanik M., SERDAR M. A., ...Daha Fazla

JOURNAL OF PERIODONTAL RESEARCH, cilt.50, sa.6, ss.766-774, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 6
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1111/jre.12263
  • Dergi Adı: JOURNAL OF PERIODONTAL RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.766-774
  • Anahtar Kelimeler: 8-hydroxy deoxyguanosine, ELISA, generalized chronic periodontitis, LC-MS, MS, oxidative stress, OXIDATIVE DNA-DAMAGE, TANDEM MASS-SPECTROMETRY, PERFORMANCE LIQUID-CHROMATOGRAPHY, LINKED-IMMUNOSORBENT-ASSAY, GINGIVAL CREVICULAR FLUID, URINARY 8-HYDROXY-2'-DEOXYGUANOSINE, ANTIOXIDANT CAPACITY, BIOMARKER EVIDENCE, PROTEIN OXIDATION, WHOLE SALIVA
  • Ankara Üniversitesi Adresli: Evet

Özet

BackgroundInflammation is associated with hydroxyl radical damage to DNA as a result of oxidative stress. 8-Hydroxy deoxyguanosine (8-OHdG) is a marker of this process and its levels in saliva could be linked to the severity of periodontal inflammation. The aim of this study was to test the sensitivity of liquid chromatography with tandem mass spectrometry (LC-MS/MS) in comparison to enzyme-linked immune sorbent assay (ELISA) for the detection of 8-OHdG in saliva in patients with chronic periodontitis before and after periodontal treatment.