Isolation and characterization of enterocin EJ97 from Enterococcus faecalis MC42
MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL, cilt.59, sa.9-10, ss.494-497, 2004 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 59 Sayı: 9-10
- Basım Tarihi: 2004
- Dergi Adı: MILCHWISSENSCHAFT-MILK SCIENCE INTERNATIONAL
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.494-497
- Anahtar Kelimeler: Enteroccocus faecalis (enterocin production), LACTIC-ACID BACTERIA, CHEESE MANUFACTURE, FAECIUM L50, STARTER, STRAIN, MILK
- Ankara Üniversitesi Adresli: Hayır
Özet
The ability of enterococci to produce bacteriocins some of which display a broad inhibitory spectrum is well known. Based on their inhibitory spectra, most of these bacteriocins have been included in the pediocin-like group. Bacteriocin-producing Enterococcus faecalis MC42 was isolated from Turkish raw milk. Bacteriocin was purified with ammonium sulphate, ion exchange and reverse phase chromatography. Amino acid sequence showed strong homology with enterocin EJ97. PCR amplification and DNA sequence analysis confirmed this result. Enterocin EJ97 was encoded on a 8.1 kb plasmid in E. faecalis MC42. Strain MC42 was sensitive to vancomycin and did not show any haemolytic activity.