Akademik Veri Yönetim Sistemi
14th International Agriculture Symposium, Sarajevo, Bosna-Hersek, 5 - 08 Ekim 2023, ss.1
Salmonellosis is defined as a bacterial infection caused by Salmonella
and is considered one of the leading causes of foodborne illness, worldwide.
Due to the high rise of antibiotic resistance in microbial populations, new and
safe natural compounds alternative to antibiotics have gained attention. This
present study aimed to evaluate the anti-microbial and anti-biofilm activities
of postbiotics of Weissella cibaria and Weissella confusa,
isolated from honey bee pollen microbiota. The anti-microbial activity of
postbiotics was tested against Salmonella enterica subsp. enterica serovar
Typhimurium ATCC 14028, serovar Enteritidis ATCC 13076, serovar Virchow ATCC
51955 and serovar Infantis ATCC 51741 using agar well diffusion assay. Minimum
Inhibitory Concentrations (MIC) of postbiotics against all pathogenic strains
were determined using microtiter plate assay, according to Clinical and
Laboratory Standards Institute guidelines. The biofilm formation ability of
each pathogen and the eradication capability of postbiotics were assessed by
crystal violet binding methodology. According to our results, postbiotics from W.
confusa displayed strong anti-microbial activity against all pathogens,
tested. Its highest anti-microbial activity was observed with an inhibition
zone of 21 ± 0.4 mm against S. enterica subsp. enterica serovar
Typhimurium ATCC 14028. MIC50 and above doses were found sufficient
to inhibit the biofilm formed by all pathogens by more than 80%. These findings
suggest that postbiotics derived from honey bee pollen microbiota could be used
as an alternative to antibiotics with their anti-microbial and anti-biofilm
activities and may play a key role in poultry.