Akademik Veri Yönetim Sistemi
European Wound Mangagement Association 2024, London, İngiltere, 1 - 03 Mayıs 2024, ss.1
Aim: This study
aims to produce electrospun nanofiber membranes containing human decellularized
skin extracellular matrix (HdsECM) for use in wound healing and skin
regeneration.
Method: Full-thickness
skin tissues were obtained from discarded tissue under the Koc University Aesthetic
and Reconstructive Surgery IRB protocol. Skin decellularization was carried out
using enzymatic protocols. A polymer solution containing 2% alginate, 7%
gelatin, and 1% HdsECM was used for membrane production. Voltage, distance, and
flow rate parameters were optimized to obtain an ideal electrospun membrane.
The porous structure was analyzed by a Scanning Electron Microscope (SEM). Mechanical
properties and degradation kinetics were investigated. Bioavailability of the
fabricated membrane was also performed using human keratinocyte cells.
Results / Discussion: An optimum nanoporous membrane
was produced using the parameters of 1.00 mm/h, 216mm, and 22 kV for
electrospinning technology. According to SEM images, it was observed that a
uniform nanofiber structure was formed in the membranes. The membrane preserved
its structural integrity for 7 days, and the keratinocyte cells survived for 14
days with their normal morphology.
Conclusion: The nanofiber electrospun membrane, including HdECM as an active agent, was tested
as a wound dressing to assess its fundamental biological and physical features.
Based on its properties, we conclude that the membrane has the potential in skin
tissue engineering for regenerative and reconstructive medicine applications.