LIVESTOCK SCIENCE, cilt.255, 2022 (SCI-Expanded)
In different artificial reproduction technologies and cloning techniques, 6-dimethylamono purine is commonly used as an activator and arrestant of miosis. Despite many reports on the effectiveness of 6-dimethylamono purine, there are reports on its deleterious effects claiming that the use of 6-dimethylamono purine is the cause of low embryo survival. To address this controversy, here, a meta-analysis on the effect of the 6-dimethylamono purine in bovine cleavage percentage, and blastocyst percentage was performed. 116 records were obtained from Scopus, Web of Science, PubMed, and Google Scholar search engines without any time and language restrictions, and 13 of them were selected as the most relevant studies. Since there were different compounds in different control groups, subgroup analysis was performed based on control group types. Our findings here showed that 6-dimethylamono purine was significantly effective in enhancing cleavage percentage (MD = 17.25 +/- 0.92, 95% CI, 15.44-19.05) and blastocyst percentage (MD = 17.98 +/- 0.63, 95% CI, 16.73-19.23). Although the subgrouping strategy could not reduce the heterogeneity within and between studies, it showed 6-dimethylamono purine had a positive effect on all subgroups except in the subgroup with a control group of non-activators, which its effect was insignificant for cleavage percentages while it had significant negative effects for blastocyst percentage. Here our findings suggest that the deleterious effects of 6-dimethylamono purine almost happen after cleavage and the low efficiency of embryo development competence in bovine relies on other activators such as ionomycin, ethanol, and inositol compared to 6-dimethylamino purine.