Hydrophilic Interaction Liquid Chromatography (HILIC): Latest Applications in the Pharmaceutical Researches
CURRENT PHARMACEUTICAL ANALYSIS, vol.17, no.3, pp.316-345, 2021 (SCI-Expanded, Scopus)
- Publication Type: Article / Review
- Volume: 17 Issue: 3
- Publication Date: 2021
- Doi Number: 10.2174/1573412916666200402101501
- Journal Name: CURRENT PHARMACEUTICAL ANALYSIS
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Biotechnology Research Abstracts, Chemical Abstracts Core, EMBASE
- Page Numbers: pp.316-345
- Keywords: Analysis, applications, HILIC, liquid chromatography, pharmaceutical, separation, TANDEM MASS-SPECTROMETRY, SOLID-PHASE EXTRACTION, LC-MS/MS METHOD, HUMAN PLASMA, RAT PLASMA, QUANTITATIVE-DETERMINATION, REVERSED-PHASE, RAPID QUANTIFICATION, ETHYL GLUCURONIDE, STATIONARY-PHASE
- Ankara University Affiliated: Yes
Abstract
Background: Significant advances have been occurred in analytical research since the 1970s by Liquid Chromatography (LC) as the separation method. Reverse Phase Liquid Chromatography (RPLC) method, using hydrophobic stationary phases and polar mobile phases, is the most commonly used chromatographic method. However, it is difficult to analyze some polar compounds with this method. Another separation method is the Normal Phase Liquid Chromatography (NPLC), which involves polar stationary phases with organic eluents. NPLC presents low-efficiency separations and asymmetric chromatographic peak shapes when analyzing polar compounds. Hydrophilic Interaction Liquid Chromatography (HILIC) is an interesting and promising alternative method for the analysis of polar compounds. HILIC is defined as a separation method that combines stationary phases used in the NPLC method and mobile phases used in the RPLC method. HILIC can be successfully applied to all types of liquid chromatographic separations such as pharmaceutical compounds, small molecules, metabolites, drugs of abuse, carbohydrates, toxins, oligosaccharides, peptides, amino acids and proteins.