Akademik Veri Yönetim Sistemi
Journal of International Advanced Otology, cilt.6, sa.2, ss.263-268, 2010 (SCI-Expanded)
Objective: Aim of this study is to investigate the respiratory tract pathogens, Chlamydia pneumoniae and Mycoplasma pneumoniae that can cause otitis media with effusion (OME) by of colonization in nasopharynx and adenoid tissue. Materials and Methods: 39 patients aged between 2 and 10 years who were submitted to myringotomy, ventilation tube insertion and adenoidectomy due to otitis media with effusion, were involved in the study. 23 patients had bilateral effusions as the rest had unilateral. Effusion and nasopharynx swab samples were obtained from patients during the surgery operation under general anesthesia. Materials were investigated by polymerase chain reaction (PCR), a molecular method for Chlamydia pneumoniae and Mycoplasma pneumoniae. Results: Chlamydia pneumoniae was detected in effusion fluids of 7 (17.9%) patients and in nasopharynx specimens among 5 (12.8%) patients. There was only 1 (2.5%) patient that Chlamydia pneumoniae is detected where in both effusion fluid and nasopharynx swab samples. For Mycoplasma pneumoniae the effusion materials were found positive with polymerase chain reaction only in 2 (5.12 %) patients and all nasopharynx swabs were negative. Conclusion: Chlamydia pneumoniae and Mycoplasma pneumoniae cannot be isolated with conventional methods. The new methods on isolation of bacteria within the samples of middle ear effusions help on planning new antibiotic treatment strategies. Copyright 2005 © The Mediterranean Society of Otology and Audiology.