Simultaneous determination of abacavir, efavirenz and valganciclovir in human serum samples by isocratic HPLC-DAD detection

Dogan-Topal B., Ozkan S. A., Uslu B.

Chromatographia, cilt.66, sa.SUPPL. 1, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 66 Sayı: SUPPL. 1
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1365/s10337-007-0302-y
  • Dergi Adı: Chromatographia
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Anahtar Kelimeler: column liquid chromatography, diode array detection, abacavir efavirenz, valganciclovir in human serum, PERFORMANCE LIQUID-CHROMATOGRAPHY, REVERSE-TRANSCRIPTASE INHIBITOR, IMMUNODEFICIENCY-VIRUS-INFECTION, HUMAN PLASMA, ANTIRETROVIRAL AGENTS, ULTRAVIOLET DETECTION, DRUG DISSOLUTION, AMPRENAVIR, SAQUINAVIR, ZIDOVUDINE
  • Ankara Üniversitesi Adresli: Evet

Özet

A rapid, sensitive, and specific reverse phase high performance liquid chromatography with diode array detection procedure for the simultaneous determination of abacavir, efavirenz and valganciclovir in spiked human serum is described. Separation was performed on a 5 μm Waters Spherisorb column (250 × 4.6 mm ID) with acetonitrile: methanol:KH2PO4 (at pH 5.00) (40:20:40 v/v/v) isocratic elution at a flow rate of 1.0 mL min -1. Calibration curves were constructed in the range of 50-30,000 ng mL-1 for abacavir and efavirenz, and 10-30,000 ng mL-1 for valganciclovir in serum samples. The limit of detection and limit of quantification concentrations of the HPLC method were 3.80 and 12.68 ng mL -1 for abacavir, 2.61 and 8.69 ng mL-1 for efavirenz, 1.30 and 4.32 ng mL-1 for valganciclovir. The method has been applied, without any interference from excipients or endogenous substances, for the simultaneous determination of these three compounds in human serum. © 2007 Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH.