Phylogenetic analysis of West Nile virus: first report of lineage 1 in donkey in Turkey

YILDIRIM Y., YILMAZ V., YAZICI K., ÖZİÇ C., ÖZKUL A., Cagirgan A. A.

TROPICAL ANIMAL HEALTH AND PRODUCTION, cilt.53, sa.5, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 5
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s11250-021-02892-z
  • Dergi Adı: TROPICAL ANIMAL HEALTH AND PRODUCTION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, Environment Index, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: Donkey, Northeast Anatolia, Phylogeny, Turkey, West Nile virus, FIELD-COLLECTED MOSQUITOS, HIGH PREVALENCE, EPIDEMIOLOGY, CIRCULATION, SEROPREVALENCE, TRANSMISSION, INFECTIONS, FLAVIVIRUS, ANIMALS, STRAINS
  • Ankara Üniversitesi Adresli: Evet

Özet

West Nile virus (WNV), a member of the Flaviviridae, is a major arbovirus that causes West Nile fever. Previous data showed the prevalence of the WNV serologically and molecular in Turkey, and the presence of lineage 1 in horses and humans has been reported. This is the first notification of partial phylogeny of WNV detected in donkeys in the northeast of Turkey (on the Iranian border). Blood serum samples collected from 25 donkeys without clinical symptoms were tested by RT-PCR. Sequence analysis of the sample detected as positive was performed. Multiple sequence alignments of reference sequences taken from GenBank were performed using the ClustalW method using the MEGA6 program. Partial nucleotide sequences of the capsid gene coding region revealed that the strains are closely related to viruses of lineage 1, clade 1a. According to the phylogenetic tree, the TUR/Igdir/donkey strain was included in the same cluster as the strain (KJ958922) previously obtained from horses in Turkey and the strain (GQ851658) from the Central African Republic. This study is the first report to show the circulation of WNV lineage 1 in donkeys in Turkey.