Molecular identification and phylogenetic analysis of spider mites (Prostigmata: Tetranychidae) of Turkey

İNAK E., Cobanoglu S., Auger P., Migeon A.

EXPERIMENTAL AND APPLIED ACAROLOGY, cilt.87, sa.2-3, ss.195-205, 2022 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 87 Sayı: 2-3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1007/s10493-022-00728-5
  • Dergi Adı: EXPERIMENTAL AND APPLIED ACAROLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, EMBASE, Environment Index, Geobase, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.195-205
  • Anahtar Kelimeler: Spider mites, Integrative taxonomy, Genetic distance, Tetranychidae, Eotetranychus, ACARI TETRANYCHIDAE, DNA-SEQUENCES, ITS2 SEQUENCES, TREE
  • Ankara Üniversitesi Adresli: Evet

Özet

The family Tetranychidae includes many agriculturally important species known as spider mites. Their morphological identification is quite difficult due to the tiny size of their taxonomic characters and the requirement for high-level expertise. This may lead to pest misidentification and thus failure in pest management. DNA-based species identification seems to offer an alternative solution to overcome these issues. In the present study, two common molecular markers-Cytochrome oxidase subunit I (COI) and Internal transcribed spacer 2 (ITS2)-were used to identify 10 spider mite species from Turkey. Furthermore, genetic distances for several of them were assessed. Panonychus ulmi and Bryobia kissophila had the lowest (1.1%) and highest (4.5%) intra-specific genetic distances, respectively. In addition, integrative taxonomy allowed to identify Eotetranychus quercicola in Turkey as a new record. The sequences herein obtained will allow rapid species identification using molecular techniques and will contribute to resolve the phylogenetic history of spider mites.