Quality assessment of frozen bull semen with the precursor A-kinase anchor protein 4 biomarker

Bastan I., AKÇAY E.

ANDROLOGIA, cilt.53, sa.9, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 53 Sayı: 9
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1111/and.14164
  • Dergi Adı: ANDROLOGIA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, EMBASE, Gender Studies Database, MEDLINE, Veterinary Science Database
  • Anahtar Kelimeler: advanced sperm analysis techniques, computer-assisted sperm analysis, proteomics, semen proteins, ASSISTED SPERM ANALYSIS, PROMISING MARKER, SPERMATOZOA, AKAP4, LOCALIZATION, EXPRESSION, PARAMETERS, PROAKAP4, MOTILITY
  • Ankara Üniversitesi Adresli: Evet

Özet

In this study, the quality of frozen bull semen was evaluated with the proAKAP4 level test. Sixty straws of frozen bull semen from various batches (n = 30) belonging to six bulls were used in the current study. The frozen bull semen samples were analysed in terms of proAKAP4 levels, sperm morphology and sperm movement parameters at hour 0 and hour 3 after thawing. The semen samples were divided into three groups according to the proAKAP4 levels: low concentration (<25 ng/10x10(6) spermatozoa), moderate concentration (25 to 39 ng/10x10(6) spermatozoa) and high concentration (>= 40 ng/10x10(6) spermatozoa). A positive correlation was found between the proAKAP4 level and total motility (TM3), progressive motility (PM3), VSL3 and VCL3 values obtained after the third-hour thermoresistance test (p < .05). There was a negative correlation between the percentage of sperm abnormal tail and the proAKAP4 level (p < .01). In addition, it was observed that the semen samples with proAKAP4 concentrations of 25 ng/10(6) spermatozoa and higher preserved the TM3 and PM3 motility characteristics. In conclusion, the proAKAP4 has the potential to become a biomarker protein to evaluate in the quality analysis of frozen-thawed semen.