Molecular analysis of delta-aminolevulinic acid dehydratase (ALAD) gene polymorphism in a Turkish population

SÜZEN H. S., Duydu Y., Aydin A.

BIOCHEMICAL GENETICS, cilt.42, sa.11-12, ss.461-467, 2004 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 42 Sayı: 11-12
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1023/b:bigi.0000043988.79625.89
  • Dergi Adı: BIOCHEMICAL GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.461-467
  • Anahtar Kelimeler: ALAD polymorphism, Turkish population, PCR-RFLP, BLOOD LEAD LEVELS, EXPOSED WORKERS, INORGANIC LEAD, SUSCEPTIBILITY, GENOTYPE
  • Ankara Üniversitesi Adresli: Evet

Özet

delta-Aminolevulinic acid dehydratase (ALAD) is a cytosolic enzyme in the heme biosynthetic pathway. ALAD is a polymorphic enzyme showing marked ethnic group differences. In this study, ALAD polymorphism is studied in a Turkish population. Genomic DNA extracted from 230 individuals and polymerase chain reaction (PCR) coupled with the restriction fragment length polymorphism (RFLP) technique were used to identify variants. The frequencies of the alleles ALAD1 and ALAD2 in Turkey were 0.887 and 0.113, respectively. This study provides the first analysis of the allele frequency distribution of the ALAD gene in a Turkish population. The results are compared with other world populations.